ANTIBACTERIAL ACTIVITY OF SWEET ORANGE (CITRUS SINENSIS) ON STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI ISOLATED FROM WOUND INFECTED

ANTIBACTERIAL ACTIVITY OF SWEET ORANGE (CITRUS SINENSIS) ON STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI ISOLATED FROM WOUND INFECTED

ABSTRACT

The antibacterial activity of sweet orange(citrus smensin) on staphylococcus aureus and Escherichia coli isolated from would infection was studied. A total number of 100 wound sample were collected form different individual from different age range within 5-8years and examined,. The test organisms S auras and E coli were cultured separately on Nutrient and macconkey agar plate and incubated for 24 hrs at 37)c. the highest isolation of bacterial organisms was obtained in staphylococcus auras as 100 (32%) followed by Escherichia coli as 92 (29%) there were isolation of other organisms, Klebsiella species was 76 (24%) followed by pseudomonas 44 (14%) the average zone of inhibition of orange extract on S aureus    was 1.8 mm and E. coli was 1.9mm also out of 100 person with S. aureus 72 showed sensitive to orange extract and out of 92 person with E coli 77 were sensitive to orange extract. The average zone of inhibition  exhibited by these drug on S. aureus were 8.95mm for Drovid 6.74 mm for ciprofloxacin while on E coli the average zones of inhibition  were 8.63mm for Drovid 6.67mm for Enythrompcin 4.47mm for anbtpicillin and 6.57mm for ciprofloxacin. The  result of the sensitivity pathen of both orange extant and antibiotics on S aureus and E coli showed that some of the isolated organisms were susceptible  while some were resistance to both orange extract and antibiotics. The result should that the zone   of inhibition of the orange extract were minimal on both S. aureus and E coli when compared to the zone of inhibition of the tested antibiotics on both organisms through the inhibition level of orange  extract invitro (outside the body) is minimal, its active ingredient vitamin  C boosts the immune system invivo (insove the body) by increasing the production of B and T cells and other white blood cells including those that destroy micro organisms.

 

TABLE OF CONTENTS

CHAPTER ONE

1.O    Introduction

CHAPTER TWO

1. Literature Review

CHAPTER THREE

1. Material and methods
   1. sterilization
   2. procurement of sweet oranges
   3. extraction of orange juice
   4. preparation  of ourture media
   5. plating technique (streak method) for the isolation of bacterial organisms.
   6. Microbial containing and microscopic work
   7. Biochemical tests for identification of bacteria’s isolates
   8. Preparation of disc or sensitivity testing o the orange extract.
   9. Sensitivity disc testing using sweet orange extract on isolated organisms.
   10. Antibiotic sensitivity disc testing on isolated bacterial organisms.

 

CHAPTER OUR

1. Result

CHAPTER FIVE

5.0     Discussion

CHAPTER SIX

1. Conclusion and recommendation
2. Reference
3. Appendix

 

LIST OF TABLES

TABLE 1

Plate court according to sex and age of population sampled.

TABLE 2

Number of groups of bacterial organisms isolated according to sex and age of population sampled.

T5ABLE 3

Perlimical test carried out for the identification of bacterial isolates

TABLE 4

Biochemical test carried out for the identification of bacterial isolates

TABLE 5

Age distribution and number of isolated organisms from both sexes of population sampled

TABLE 6

Age distribution number and percentage occurrence of staphylococcus auraus Escherichia coli Klebsiella and pseudomonas

 

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ANTIBACTERIAL ACTIVITY OF SWEET ORANGE (CITRUS SINENSIS) ON STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI ISOLATED FROM WOUND INFECTED