ANTISICKLING POTENTIALS OF OCCIMUM BASILICUM AND VERNONIA AMYGDALINA LEAF EXTRACTS ON SICKLE CELL DISEASE

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ABSTRACT

Experiment was carried out in the department of Genetics and Biotechnology to evaluate the antisickling activity of two local plants (Vernonia amygdalina and Ocimum basilicum). Blood was gotten from six (6) sickle cell patients ranging from six (6) to twenty five years (25) that were not at crisis. Blood(0.5ml)was mixed with sodium metabisulphite salt and aqueous extract of plant materials to evaluate the inhibition and reversal time.        Result showed that at (10mg/ml) concentration, Ocimum basilicum inhibited 47 cells while Vernonia amygdalina inhibited 45 cells after five minutes. Furthermore at the same concentration and time, Ocimum basilicum reversed 23 cells while Vernonia amygdalina reversed 20 cells. It was thus concluded that Ocimum basilicum was effective over Vernonia amygdalina in the management of sickle cell disease and its implication in drug development.                                                

TABLE OF CONTENTS

TITLE PAGE

CERTIFICATION

DEDICATION

ACKNOWLEDGMENTS

ABSTRACT

TABLE OF CONTENTS

CHAPTER ONE: INTRODUCTION

1.1    Background of Study

1.2   Justification

1.3   Objectives

CHAPTER TWO: LITERATURE REVIEW

2.1       Background and Traditional Application of Vernoniaamygdalina.

2.2.      Bioactivities of Vernonia amygadalina

2.2.1   Anti Bacterial Activities Of Vernonia amygdalina

2.2.2    Antifungal Activities Of Vernonia amygdalina

2.2.3   Antiviral Activity of Vernonia amygdalina

2.2.4    Antimutagenic Properties Of Vernonia amygdalina

2.2.5    Anti parasitic activity of Vernonia amygdalina

2.3.      Bioactivities of Ocimum basilicum

2.3.1    Rich in Antibacterial properties

2.3.2   Rich In Antioxidant

2.3.3    Reduce Inflammation And Swelling

CHAPTER THREE: MATERIALS AND METHODS

3.1       Experimental site

3.2       Source of Experimental materials:

3.3       Collection and Preparation of Blood Sample

3.4       Preparation for plant materials

3.5       Extraction

3.6.      Bioassay of Plants Extract for Antisickling Activity

3.7    Experimental Design/Layout

3.8    Statistical Analysis

CHAPTER FOUR: RESULTS AND DISCUSSION

4.1       Result

4.2       Discussion

CHAPTER FIVE: SUMMARY, CONCLUSION AND RECOMMENDATION

5.1       Summary

5.2       Conclusion

Recommendation                                                                                                       

REFERENCES

APPENDIX