ABSTRACT
The study was carried out to determine the effect of administration of ethanol leaves extracts of Mangiferaindica and Gongronemalatifolium on serum albumin and globulin levels in normal albino wistar rats. Sixteen (16) male albino wistar rats (185 – 221g) were randomly assigned four groups of four rats each. Groups 1 and 3 were treated with 200mg/kg Mangiferaindicaand Gongronemalatifoliumleaves extracts respectively, and group 2 was treated with 200mg/kg of the combined extracts at 50:50 dosage ratio, while the remaining group served as control without extract administration. All animals were allowed free access to commercial rat mash with distilled water throughout the 21 days of extracts administration. The results of mean serum albumin levels in the treated group recorded significant increase when compared to the control (P < 0.05). No significant difference was observed in the treatment groups 2 and 3 when compared to group the control (P > 0.05). Non significant increase in serum albumin levels was seen in groups 2 and 3 when compared to group 1 (P > 0.05). Group 3 recorded significant decrease in the mean serum globulin levels when compared to group 1 and 2 (P < 0.05), and when compared to the control (P < 0.05). Group 1 showed no significant difference in serum globulin level when compared to the control (P > 0.05). Significant decrease in group 2 was observed when compared to the control (P < 0.05). However, the implications of these results are well discussed.
Keywords:Mangiferaindica, Gongronemalatifolium, albumin, globulin, albino rats.
TABLE OF CONTENT
CONTENTS PAGE
TITLE PAGE – – – – – – – I
CERTIFICATION – – – – – – II
DEDICATION – – – – – – – III
ACKNOWLEDGEMENTS – – – – – IV
ABSTRACT – – – – – – V
TABLE OF CONTENTS – – – – – – VI
CHAPTER ONE: INTRODUCTION – – – 1
1.1 Background of the Study – – – – – 1
1.2 Aim and Objectives of the Study – – – – 5
1.2.1 Aim of the Study – – – – – – 5
1.2.2 Objectives of the Study – – – – – 6
1.3 Scope and Limitations of the study – – – 6
CHAPTER TWO: LITERATURE REVIEW – – 7
- Description of the Plants – – – – – 7
- Description of Mangiferaindica Plant – – – 7
- Description of GongronemalatifoliumPlant – – 9
- Classification of the Plants – – – – 10
- Classification of Mangiferaindica Plant – – 10
- Classification of GongronemalatifoliumPlant – 11
- Medicinal values of the Plants – – – – 12
- Medicinal value of Mangiferaindica Plant – – 12
- Medicinal value of GongronemalatifoliumPlant – 19
- Proteins and their Biochemical Functions – – 24
- Globulin and Biochemical Functions – – – 24
- Albumin and Biochemical Functions – – – 26
- Effects of Phytochemicals in Human Health. – – 29
- Phytochemical Composition of Mangifera
indica Plant – – – – – – – 29
- Phytochemical Composition of Gongronema
latifolium Plant – – – – – – 31
CHAPTER THREE: MATERIALS AND METHODS – 34
3.1 Material and Reagents – – – – – 34
3.1.1 Collection and Preparation of the Plant Samples – 35
3.1.2 Experimental Design, Grouping and
Treatment of Animal – – – – – 36
3.1.3 Collection and preservation of blood Samples – 37
3.2 Methods – – – – – – – 38
3.2.1 Determination of Serum Globulin – – – 38
3.2.2 Determination of Serum Albumin – – – 39
3.3 Statistical Analysis – – – – – – 40
CHAPTER FOUR: RESULTS AND DISCUSSION – 41
4.1 Results – – – – – – – 42
4.2 Discussion – – – – – – – 42
CHAPTER FIVE: CONCLUSION AND
RECOMMENDATIONS – – – – – 48
5.1 Conclusion – – – – – – – 48
5.2 Recommendations – – – – – – 48
References