HYPOLIPIDEMIC EFFECT OF TIGERNUT (Cyperus esculentus) ON ALBINO RATS
The effect of intake of tigernut extract was studied. Doses of 0.2ml and 0.8ml of tigernut (cyperus esculentus) extract as well as the administration of 0.3ml questran (hypolipidemic drug) were orally administered to 6 weeks old male rats for 21 days. The effect of this administration on serum lipid levels such as cholesterol, triglyceride, HDL-cholesterol and LDL-cholesterol were studied. The result obtained indicate that tigernut causes significant (P<0.05) decrease in the cholesterol, triglyceride and LDL-cholesterol and a significant (p<0.05) increase in the HDL and so exhibit hypolipidemic properties.
Title page                           i
Certification page                     ii
Dedication                           iii
Acknowledgement                     iv
Abstract                           v
Table of contents                     vi
List of figures                        x
CHAPTER ONE:
1.0   Introduction                     1
1.1   Background of the study               1
1.2   Statement of problem               4
1.3   Objective of the study               4
1.4   Hypothesis or research questions         5
1.5   Significance of the study               5
CHAPTER TWO:
2.0   Literature Review                     6
2.1   History of Tigernut                     6
2.2   Uses of Tigernut                      7
2.3   Health benefits of Tigernuts               7
2.4   Nutritional value of Tigernut               11
2.5   Lipids and its effects                     12
2.6   Effect of Tigernut on metabolism            14
CHAPTER THREE
3.0   Materials and method                     16
3.1   Material                           16
3.2   Chemical Reagents                     16
3.3   Methods                           17
3.3.1      Collection of sample                  17
3.3.2      Extraction of Tigernut oil               17
3.3.3      Care and Management of experimental animal   18
3.3.4      Experimental design                  18
3.3.5      Collection of blood samples for Biochemical  analysis                  20
3.3.6      Determination of cholesterol            20
3.3.7      Determination of triglyceride            22
3.3.8      Determination of HDL cholesterol         24
3.3.9      Determination of LDL cholesterol          26
CHAPTER FOUR:
4.0   Results                           27
CHAPTER FIVE
5.0   Discussion, Conclusion and Recommendation      33
5.1   Discussion                           33
5.1.1   Effect of tigernut extract on serum cholesterol of rats                  33
5.1.2   Effect of Tigernut extract on serum triglyceride of rats                  34
5.1.3   Effect of Tigernut extract on serum HDL of rats   34
5.1.4   Effects of Tigernut on serum LDL of rats      34
5.1.5   Effect of Tigernut extract on body weight of rats   35
5.2   Conclusion                           35
5.3   Recommendation                     36
References                                 7
Appendix                                40
LIST OF FIGURES
figure 1:      Serum cholesterol concentration    28
figure II: Â Â Â Serum triglyceride concentration 29
figure III:   Serum HDL-C concentration       30
figure iv:          Serum LDL-C concentration       31
figure v:          Percentage increase in body weight   32
