MICROBIOLOGY AND PHYSICOCHEMISTRY OF GREAT QUA RIVER

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ABSTRACT

The environmental quality is greatly focused on water because of its importance in maintaining the human health and health of the ecosystem. Many parts of the world are facing water scarcity problem due to limitation of water resources coincided with increasing population. Rivers are vital freshwater systems that are critical for the sustenance of life. Microbiology and physicochemical parameters of qua river was analyzed, the results revealed that pH ranged from 6.37 to 6.45 with mean temperature 23.42 to 23.90 °C. For water sample, and for sediment sample the pH range from 6.86 to 6.89.

The results obtained showed that the sediment samples had a significantly higher counts of 2.22±2.54±2.36×107 cfug-1total heterotrophic bacteria (THB), than water sample (1.22± 1.78 ±1.90× 106cfug-1 and 2.46 ± 2.20 × 106 cfug-1) respectively. The total fungal (TF) densities observed for sediment where 1.44 ± 1.20±1.08 x 104 cfug-1 and 9.8 ± 7.8±9.2 x 103 cfug-1 for water samples. Other physicochemical parameters including, biochemical oxygen demand Investigations are based on different parameters, EC, TDS, TSS, DO, COD, total alkalinity, total acidity, chlorides, free CO2, hardness, nitrates, phosphates, sulphates, The results obtained also indicate that the sediment samples show a remarkable variation in physicochemical parameters. microbial characterization Results of Bacteriological analyses such as total heterotrophic count, total fungi count, coliform counts revealed high degree of faecal pollution of the river., bacteria species and  fungi species were found at the end of the microbial analysis we. It’s inferred that the Qua River is polluted, bacteriologically contaminated and unsafe for human and animal consumption.

Key words: aquatic pollution, bacteriological quality, water contamination, surface water monitoring and biosafety.

TABLE OF CONTENTS

CHAPTER ONE: INTRODUCTION

1.1 Background to study

1.2 Aim and Objectives of the study

1.3 Significance of the study

CHAPTER TWO: LITERATURE REVIEW

2.1 Brief history of the Qua River and its location

2.2 water

2.2.1 Sources of water

2.2.2 General uses of water

2.3 Physical qualities of water

2.3.1 Biological parameters

2.3.2 Indicator organisms

2.3.3 water contamination

2.3.4 Open deification in river bodies

2.3.5 Diseases associated with water

2.4 Water quality requirements standard and public health roles

2.4.1 Water treatment processes

2.5 Aquatic microbial ecology

2.5.1 Factors affecting microorganisms in freshwater ecosystem

2.5.2 Physicochemical characteristics of freshwater ecosystem

CHAPTER THREE: MATERIALS AND METHODS

3:1 Study site

3.2 Sample collection

3.2.1 Pretreatment of samples

3.2.2 Sample preparation

3.2.3 Microbiological analysis

3.2.4 Estimation of microbial densities of the water and sediment sample

3.2.5 Isolation, purification and maintenance of pure microbial isolates

3.2.6 Characterization and identification of bacterial isolates

3.2.7 Characterization and identification of fungi isolates

3.2.8 Physicochemical analysis of the water and sediment samples

3..2.9 Experimental design and data analysis

4.1 Total heterotrophic bacteria count for surface water across the sampling stations

4.2 Total fungi count for surface water across the sample stations

4.3 Total heterotrophic bacteria count for sediment across the sample stations

4.4 Total fungi count for sediment sample across the sample stations

4.5 Physicochemical analysis of surface water sample across the stations

4.6Physicochemical parameters of Sediment sample across the stations

5.1 Discussion

5.2 conclusion

5.3 Recommendations