ABSTRACT
The process of developing reliable and environmentally friendly metallic nanoparticles (NPs) holds significant importance in the biomedical application of nanotechnology. Zinc oxide nanoparticles (ZnONps) and silver nanoparticles (AgNps) have garnered attention in the field of nanobiomedicine due to their biocompatibility and antimicrobial properties. This study aims to assess the eco-friendly synthesis of ZnONps, silver-doped silver nanoparticles, and zinc oxide nanoparticles, along with their antimicrobial characteristics.
The synthesis of ZnONps and AgNps using Senna occidentalis, a plant-based method, was carried out. The synthesized ZnONps and AgNps underwent characterization through techniques such as UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FT-IR), Energy Dispersive Spectrum (EDS), and Transmission Electron Microscope (TEM) analysis. To evaluate their antimicrobial efficacy, agar well diffusion assays were conducted.
The findings revealed that the synthesized ZnONps and AgNps exhibited a particle size of 52.50 nm with an intensity of 13.85% at a wavelength of 393 nm. In contrast, AgNps exhibited a distribution around 83.40 nm with an intensity of 14% at a wavelength of 331 nm. The TEM analysis depicted that AgNps exhibited a highly aggregated spherical morphology, while ZnONps displayed an irregular shape. The EDS Spectrum of AgNps indicated an optical absorption band peak at 3.0-3.5 KeV, while ZnONps exhibited peaks at 1.0, 8.8, 9.8 KeV for pH levels 7, 10, 11, and 12. The FT-IR spectra of bio-synthesized AgNps and ZnONps exhibited signals at 2893 and 3493 cm-1, corresponding to the –O-H stretching vibrations.
Regarding antimicrobial activity, AgNps demonstrated a dose-dependent increase in inhibitory effects against Streptococcus pyogenes, Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The doped zinc and silver nanoparticles displayed inhibitory effects only against S. pyogenes among the tested organisms. ZnONps exhibited limited antibacterial activity against S. aureus alone. In conclusion, Senna occidentalis facilitated the reduction of zinc acetate and silver nitrate to yield zinc oxide and silver ions, respectively. These compounds exhibited a moderate inhibitory effect on the selected microorganisms.
ANTIMICROBIAL EFFECTS OF ZINC OXIDE AND SILVER NANOPARTICLES SYNTHESIZED FROM PLANT SOURCES WITH AND WITHOUT DOPING. GET MORE, ACTUARIAL SCIENCE PROJECT TOPICS AND MATERIALS
