PRELIMINARY PHYTOCHEMICAL AND ANTIMICROBIAL SCREENING OF FLOWER AND STALK OF MANGIFERA INDICA (OPIORO MANGO)
TABLE OF CONTENT
Title page
Declaration
Certification
Dedication
Acknowledgment
Table of content
Abstract
CHAPTER ONE
1.0INTRODUCTION
OBJECTIVE OF THIS RESEARCH
1.1 LITERATURE REVIEW
1.2Â Â Â GENERAL USES AND IMPORTANCE OF
MANGIFERA INDIC
1.3 MORPHLOGY OF MANGIFERA INDICA; THE TREE AND ITS
FLOWERS
CHAPTER TWO
2.0 MATERIALS AND METHODS
2.1MATERIALS
2.2EQUIPMENTS AND APPARATUS
2.3 CHEMICAL REAGENTS
2.4 PHYTOCHEMICAL SCREENING (QUANTITATIVE ANALYSIS)
2.5 DETERMINATION OF SAPONIN
2.6 DETERMINATION OF ALKALOID
2.7 DETERMINATION OF TANINS
2.8 DETERMINATION OF MOISTURE CONTENT
2.9 DETERMINATION OF TOTAL ASH
2.10 CARDIAC GLYCOSIDE
2.11 TEST FOR ANTHRACENE GLYCOSIDES
2.12TERPENOIDS
2.13Â pH AT 200C (10%)
CHAPTER THREE
TABLE 3.1 ORGANOLEPTICÂ TESTÂ RESULT
TABLE 3.2 RESULT OF THEÂ PHYTOCHEMICALÂ ANALYSIS
OF FLOWER AND STALK OF Mangifera Indica
TABLE 3.3RESULT OF ANTI-BACTERIAL ACTIVITY OF TWO
GRAM POSITIVE BACTERIA IN TWO CRUDE
EXTRACTS OF FLOWER AND STALK OF
Mangifera indica
TABLE 3.4 RESULT OF ANTI-BACTERIAL ACTIVITY OF
TWO GRAM NEGATIVE BACTERIA IN TWO
CRUDE EXTRACTS OF FLOWER AND STALK
OF Mangifera indica
TABLE 3.5 RESULT OF ANTI-FUNGAL ACTIVITY OF
TWO FUNGI IN TWO CRUDE EXTRACTS
OF FLOWER AND STALK
OF Mangifera indica
TABLE 3.6 RESULT OF MINIMUM INHIBITORY
CONCENTRATION
(MIC) OF CRUDE EXTRACT OF FLOWER
AND STALK
OF Mangifera indica
CHAPTER FOUR
DISCUSSION
CONCLUSION
RECOMMENDATION
REFERENCES
APPENDIX
ABSTRACT
Phytochemical and antimicrobial screening analysis of Mangifera indica indicated that the presence of the following moisture content 12.33% +0.0556%, Alkaloid     7.35% +0.0291%, Tannins 18.22% +0.0070%, Saponic Glycoside 3.82% +0.0264%, Flanonoid 6.11% +0.0158%, Phenolic glycoside 0.010% + 0.0012%, Terpenoids 5.71% + 0.01%, Antrachionic 10.59% + 0.0158%, pH 3.84% + 0.0529%. Cardiac Glycoside 7.90% + 0.0234%. Extracts of Mangifera indica were investigated for its antifungal and antibacterial activities. The aqueous extract did not inhibit any of the test fungi, the ethanolic extract produced zones of inhibition against Staphylococcus aureus, S. pyogens, Escherichia coli, Bacilus subfilis, while C. albican and a. flavus were not inhibited.
